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BPP702S-BIOCHEMISTRY- BIOCHEMICAL PRINCIPLES AND PRACTICE-JAN 2020 |
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p
NAMIBIA UNIVERSITY
OF SCIENCE AND TECHNOLOGY
FACULTY OF HEALTH AND APPLIED SCIENCES
DEPARTMENT OF NATURAL AND APPLIED SCIENCES
QUALIFICATION: BACHELOR OF SCIENCE
QUALIFICATION CODE: 07BOSH
LEVEL: 7
COURSE NAME: BIOCHEMISTRY: BIOCHEMICAL | COURSE CODE: BPP702S
PRINCIPLES AND PRACTICE
SESSION: JANUARY 2020
DURATION: 3 HOURS
PAPER: THEORY
MARKS: 100
SUPPLEMENTARY/SECOND OPPORTUNITY EXAMINATION QUESTION PAPER
EXAMINER
DR LAMECH MWAPAGHA
MODERATOR: | DR PETRINA KAPEWANGOLO
INSTRUCTIONS
Answer ALL the questions.
Write clearly and neatly.
Number the answers clearly.
All written work MUST be done in BLUE or BLACK ink.
PERMISSIBLE MATERIALS
None
THIS QUESTION PAPER CONSISTS OF FIVE (5) PAGES
(Including this front page)
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QUESTION 1
[15]
a) Calculate the pKa of lactic acid, given that when the concentration of free lactic acid is 0.01M
and the concentration of lactate is 0.087M, the pH is 4.80
(4)
b) Given the structure of aspartic acid and the pKa values as shown below
H
H
| ,O
\\
7
N—-C—C
H =| oH
CH
|2
Lo.
HO
O
pKa1=1.88
pKa2=3.65
pKa3=9.68
Determine the pl value of aspartic acid and show clearly how you arrive at the answer
(6)
c) Briefly discuss the mechanism of surface tension as one of the chemical reactions taking place
in living matter, in vivo
(5)
QUESTION 2
[15]
a) Use the structures of the following amino acids to classify these compounds as either
nonpolar/hydrophobic, polar/hydrophilic, negatively charged/hydrophilic, or positively
charged/hydrophilic
(4)
|. Valine: R = -CH(CH3)2
Il. Serine: R = -CH20OH
Ill. Aspartic acid: R = -CH2CO2H
IV. Lysine: R = -(CH2)4NH3
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b) State FIVE (5) main functions of the amino acids Valine
(5)
c) Discuss THREE (3) important parameters to consider during the planning of an ion
exchange-based separation
(6)
QUESTION 3
[16]
a) Two different disaccharides are shown in the figure below
“CH,OH
0
Hf
‘
HO
°CH,OH
Sina
OH
H
H OH
H OH
Maltose
Sucrose
Name the monosaccharides that these disaccharides are composed of indicating their
stereoisomer form
(2)
b) Draw the structures and name the two stereoisomer monosaccharides that form when the
disaccharide below is hydrolyzed
(4)
CH OH
z
c) Give a detailed description of how a DNA molecule is translated into a protein
(10)
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QUESTION 4
[12]
a) Briefly describe the following mutations;
(4)
]. | Nonsense Mutation
Il. Frame shift Mutation
III.
Misense Mutation
IV. Silent Mutation
b) Distinguish between Positive and Negative Allosterism
(4)
c) The Michaelis-Menton equation is frequently rearranged to its reciprocal form to simplify
plots to a form known as the Lineweaver-Burk plot. Draw a Lineweaver-Burk plot with a
single line, and indicate the values generally plotted on the X and Y axes as well as the
values generally located at the X and Y intercepts
(4)
QUESTION 5
[14]
a) Outline the FOUR (4) enzyme sites that regulate gluconeogenesis
(4)
b) Using structural formulas, write the balanced chemical equation for the reactions where
GTP is produced in the Kreb cycle
(4)
c) Briefly describe the anabolic role of the TCA cycle in fatty acid synthesis
QUESTION 6
[11]
a) Describe THREE (3) types of membrane proteins
(3)
b) Discuss THREE (3) characteristics of the genetic code
(3)
c) Give the possible symptoms of deficiency of the following vitamins
(5)
|. Vitamin Bz (Riboflavin)
Il. Vitamin Biz
Ill. Vitamin A (Retinol)
IV. Vitamin K (Phylloquinone)
V. Vitamin D
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QUESTION 7
,
[17]
a) Outline FOUR (4) likely reasons of poor drug absorption according to Lipinski’s rules
(4)
b) Elaborate on how Inositol triphosphate (IP3) and diacylglycerol (DAG) with the
aid of Phospholipase C modulates signal transduction
(5)
c) Discuss signal transduction
(8)
THE END