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AMB821S - ADVANCED MICROBIOLOGY - 2ND OPP - JANUARY 2024 |
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n Am I BI A u n IVER s ITY
OF SCIEnCE AnDTECHnOLOGY
Faculty of Health, Natural
Resources and Applied
Sciences
School of Health Sciences
Department of Clinical
Health Sciences
13 Jackson Kaujeua Street
Private Bag 13388
Windhoek
NAMIBIA
T: +264 612072970
F: +264 61 207 9970
E: dchs@nust.na
W: www.nust.na
QUALIFICATION: BACHELOR OF SCIENCESHONOURS
QUALIFICATION CODE: 08BOSH
COURSE:ADVANCED MICROBIOLOGY
DATE: JANUARY 2024
DURATION: 3 HOURS
LEVEL:8
COURSECODE: AMB821S
SESSION: 1
MARKS: 100
SUPPLEMENTARY/SECOND OPPORTUNITY EXAMINATIONS: QUESTION PAPER
EXAMINER:
MODERATOR:
Dr Munyaradzi Zivuku
Prof Jane Misihairabgwi
INSTRUCTIONS
1. Answer all questions.
2. Each question must be answered on the separate answer sheet.
3. Please write neatly and legibly.
4. Do not use the left side margin of the exam paper. This must be allowed for the
examiner.
5. No books, notes and other additional aids are allowed.
6. Mark all answers clearly with their respective question numbers.
PERMISSIBLE MATERIALS
1. Non-Programmable Calculator
This paper consists of 4 pages including this front page
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QUESTION 1 (20}
1. 1 In 1872, Frans Schrandinger proposed that E.coli could be used to indicate that water
is contaminated with feces.Briefly outline the main reason why E. coli was chosen as
a test microorganism for the presence of contaminants in feces.
(5)
1.2 Propose a method that could be used for the isolation and enumeration of coliforms
from contaminated water.
(5)
1.3 Write short notes on the preservation and maintenance of microorganisms
(10)
QUESTION 2 (20)
2.1 Jane is fourth year B.Sc. Honours student and she did an experiment to analyse the
microbial load of water samples using the Most Probable Number (MPN) method in
her laboratory. Jane used the MPN table (illustrated in table 1) and got an MPN of 9.2
per 100 ml.
Table 1: Table ofthe most Probable Number (MPN) per 100ml of sampling using three
tubes of each dilution
Number of positive tubes in dilutions
10ml
1ml
0.1ml
MPN per 100 ml
0
0
0
0
1
0
3
0
0
3
6
0
1
0
3
0
1
1
6.1
0
1
2
9.2
0
1
3
12
AdvancedMicrobiology (AMB821S)
1' 1 Opportunity November 2023
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Briefly describe the principle and procedure of MPN that she used to arrive at an MPN
of 9.2 per 100 ml.
(8)
2.2 What is the disadvantages of MPN method as a diagnostic tool in microbiological
samples.
(3)
2.3 Outline the principle of protein evolution in industrial microbiology.
(9)
SECTION B (60 MARKS)
QUESTION 3 (20)
3.1 Differentiate between transcriptomes and proteomes.
(4)
3.2 Explain the importance of measurements of gene expression.
(6)
3.3 Discuss how infectious diseases such Ebola virus can be prevented and
Controlled.
(10)
QUESTION 4 (20)
4.1 Briefly describe three factors for the control and optimization of a bioremediation
process.
(6)
4.2 For a chosen bioremediation, describe how pollution gets into the environment. (3)
Answer
4.3 With the aid of an annotated diagram, discuss the role of antigen presenting cell in
cell mediated immunity following the entering of infectious agent in the human body
and its eventual removal from the body.
(10)
Advanced Microbiology (AMB 821S)
1'1 0pportunity November 2023
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QUESTION S (20)
5.1 Briefly explain the conditions necessary for a pathogen to cause disease.
(5)
5.2 The occurrence of plasmids in microorganism is a necessary evil. Discussthe
statement.
(5)
5.3 Outline the pathogenic properties of viruses.
(5)
5.4 Give an account of the application of amylases enzymes in the food industry.
(5)
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END OF THE QUESTION PAPER
Advanced Microbiology (AMB 821S)
1'10pportunity November 2023
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