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MIB701S - MICROBIOLOGY - 2ND OPP - JULY 2023 |
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nAm I BIA un IVE RSITY
OF SCIEnCE Ano TECHnOLOGY
FACULTYOF HEALTH,NATURALRESOURCESAND APPLIEDSCIENCES
SCHOOLOF NATURALAND APPLIEDSCIENCES
DEPARTMENTOF BIOLOGY,CHEMISTRYAND PHYSICS
QUALIFICATION: BACHELOROF SCIENCE
QUALIFICATION CODE: 07BOSC
LEVEL: 7
COURSECODE: MIB701S
COURSENAME: MICROBIOLOGY
SESSION:JULY 2023
DURATION: 3 HOURS
PAPER:THEORY
MARKS: 100 MARKS
SECOND/SUPPLEMENTARY EXAMINATIONS OPPORTUNITY QUESTION PAPER
EXAMINER(S) DR. MUNYARADZI ZIVUKU
MODERATOR: PROF.JANE MISIHAIRABGWI
INSTRUCTIONS
1. There are FIVE questions on this paper. Answer ALL the questions.
2. The number of marks are given in brackets ( ) at the end of each
question or part question.
3. Write clearly and neatly.
4. Number the answers clearly.
THIS QUESTION PAPERCONSISTSOF 3 PAGES(Including this front page)
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SECTION A
(40)
QUESTION 1 (20)
1.1 Several theories have been postulated to try and explain whether an
HIV virus is a living entity or not. From what you have studied, briefly
discuss whether the HIV virus is a living or non-living entity? Give
reasons to justify your answer.
(5)
1.2 Discuss, why in the early 1860s, scientist erroneously placed bacteria
and fungi under the Kingdom Plantae.
(5)
1.3 Evaluate the use of positive and negative staining techniques in the
identification and classification of bacteria.
(10)
QUESTION 2 (20)
2.1 Distinguish between endotoxins and exotoxins.
(2)
2.2 Briefly discuss how the following factors influence microbial death.
(i) Time of exposure.
(2)
(ii) Environmental factors.
(2)
(iii) Number and type of microorganism present.
(2)
2.3 Briefly discuss the viable plate count method for the enumeration
of bacteria.
(12)
SECTION 8
(60)
QUESTION 3 (20)
3.1 Describe the principle on which the Gram stain is based.
(3)
3.2 Explain why the oil immersion objective lens (marked with black rings)
are immersed in oil during use.
(3)
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3.3 What is a fastidious microorganism?
(2)
3.4 How does the microbiologist overcome the challenges of culturing
fastidious microorganism with examples
(3)
3.5 Discuss the exchange of genetic information in bacteria.
(9)
QUESTION 4 (20)
4.1 Give an account of how microorganisms are used for the extraction
of low-grade ores.
(10)
4.2 Discuss the role of microorganisms in sewage and water
treatment.
(10)
QUESTION 5 (20)
5.1 "Bacteria shows a great deal of diversity in their nutritional
patterns". Discuss this statement.
(20)
END OF QUESTION PAPER
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