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MRT811S- METHODS IN RECOMBINANT DNA TECHNOLOGY - 1st Opp - JUNE 2022 |
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NAMIBIA UNIVERSITY
OF SCIENCE AND TECHNOLOGY
FACULTY OF HEALTH, APPLIED SCIENCES AND NATURAL RESOURCES
DEPARTMENT OF NATURAL AND APPLIED SCIENCES
QUALIFICATION: BACHELOR OF SCIENCE HONOURS
QUALIFICATION CODE: 08BOSH
LEVEL: 8
COURSE CODE: MRT811S
COURSE NAME: METHODS IN RECOMBINANT DNA
TECHNOLOGY
SESSION: JUNE 2022
DURATION: 3 HOURS
PAPER: THEORY
MARKS: 100
EXAMINER
FIRST OPPORTUNITY QUESTION PAPER
DR LAMECH MWAPAGHA
MODERATOR | DR RONNIE BOCK
INSTRUCTIONS
Answer ALL the questions.
Write clearly and neatly.
Number the answers clearly.
All written work MUST be done in BLUE or BLACK ink.
PERMISSIBLE MATERIALS
None
THIS QUESTION PAPER CONSISTS OF THREE (3) PAGES
(Including this front page)
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Question 1
[10]
a) What were the main goals of the Human genome project?
(4)
b) Distinguish between a genome map and a genome sequence.
(2)
c) Briefly elaborate on how the knowledge of human genetic variation can be utilised in
promoting health and combating disease.
(4)
Question 2
[17]
a) State TWO (2) ways in which genes can be transferred in DNA recombination technology, and
for each give TWO (2) examples.
(6)
b) Mention THREE (3) demerits of the Retrovirus-mediated Gene Transfer
(3)
c) RNA splicing is a form of RNA processing in which a newly made precursor messenger RNA (mRNA)
is transformed into a mature RNA by removing the non-coding sequences termed introns.
Describe RNA alternative splicing.
(8)
Question 3
[11]
a) Compare and contrast the One- and Two-Channel DNA microarray hybridisation
approaches.
(4)
b) What are the THREE (3) major steps you would follow in order to identify a peptide of interest
using the Peptide mass finger printing technique.
(3)
c) Discuss FOUR (4) important tools providing investigators with sensitive and specific means
of identifying and characterizing proteins.
(4)
Question 4
[16]
a) DNA ligase is used for ligation of foreign DNA and vectors during gene cloning. Briefly explain the
mechanism of DNA ligase in the enzymatic reaction of DNA ligation.
(5)
b) Describe the mechanism and purpose of dephosphorylation in cloning.
(3)
c) Distinguish between the following types of vectors;
(8)
|. Shuttle Vectors;
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Il. | Plasmid Vectors;
lll. Retrovirus Vectors;
IV. Cosmid Vectors;
Question 5
[18]
a) What are some of the concerns that scientists grapple with when it comes to animal cloning? (4)
b) Therapeutic cloning involves creating a line of embryonic stem cells genetically identical to an
individual. These stem cells can then be used in experiments aimed at understanding disease and
developing new treatments for disease. In line with the above description outline the steps
involved in therapeutic cloning.
(10)
c) Give a description of the nucleic acid probe technique as used in DNA recombination
technology.
(4)
Question 6
[12]
a) The Particle bombardment device (gene gun), was developed to enable penetration of the cell wall
so that genetic material containing a gene of interest can be transferred into the cell. Briefly
describe the mechanism of action of the gene gun
(4)
b) State FOUR (4) ethical issues associated with human cloning.
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(4)
c) Give FOUR (4) differences between the Ti and Ri Plasmids
(4)
Question 7
[16]
a) Discuss FOUR (4) applications of metabolomics:
(8)
b) State FOUR (4) limitations of metabolomics:
(4)
c) What are some of the ethical arguments for and against the cloning of humans?
(4)
THE END
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