SECTION A
QUESTION 1
(42 MARKS)
1.1 Explain the major changes that may occur in food sample before analysis and
may result in wrong results.
(8)
1.2 Explain the following terms used in establishment of research project.
1.2.1 Sampling Plan.
(2)
1.2.2 Compartmentalised population.
(2)
1.2.3. Continuous population.
(2)
1.3 Outline three (3) major types of chromatographic methods used in analysis of
carbohydrates.
(3)
1.4 In the experiment to determine the concentration of reducing sugars in a food sample
using the Thin Layer Chromatography, the solvent moved a distance 1 cm on the
stationary phase and the two categories of reducing sugars (A and B) in the sample,
each moved 0.3 cm and 0.6 cm respectively.
1.4.1 Calculate the retention factor (Rr) of the sample.
(4)
1.4.2 Using the Rr value explain the difference between A and B.
(5)
1.5 A found sample was weighed in crucible and the weight recorded as 30 kg. It was
dried in the oven over 24 hrs period and weight of the dried sample was 25 g. If the
weight of the crucible before oven drying was 1.8 g.
1.5.1 Determine the moisture content (%) of the sample.
(3)
1.5.2 Determine the total solids (%) of the sample.
(3)