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PAB811S - PLANT AND ANIMAL BIOTECHNOLOGY - 2ND OPP - JULY 2022 |
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NAMIBIA UNIVERSITY
OF SCIENCE AND TECHNOLOGY
FACULTY OF HEALTH, APPLIED SCIENCES AND NATURAL RESOURCES
DEPARTMENT OF NATURAL AND APPLIED SCIENCES
QUALIFICATION: BACHELOR OF SCIENCE HONOURS
QUALIFICATION CODE: O8BOSH
COURSE CODE: PAB811S
LEVEL: 8
COURSE NAME: PBLIAONTTECAHNNDOLAONGIYMAL
SESSION: JULY 2022
DURATION: 3 HOURS
PAPER: THEORY
MARKS: 100
SUPPLEMENTARY / SECOND OPPORTUNITY EXAMINATION QUESTION PAPER
EXAMINER(S) | DR JEYA KENNEDY
MOpERATOR: | DRJUAN VORSTER
INSTRUCTIONS
Write clearly and neatly
Number the answers clearly
All written work MUST be done in blue or black ink
No books, notes and other additional aids are allowed
Mark all answers clearly with their respective question
Draw diagrams wherever necessary
numbers
PERMISSIBLE MATERIALS
None
THIS QUESTION PAPER CONSISTS OF 6 PAGES
(Including this front page)
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QUESTION 1:
Multiple choices
[8]
Lvl Somatic hybridization is achieved through;
(1)
a) micropropagation
b) recombination DNA technology
c) protoplast fusion
d) electrofusion
La
What is a cell line?
(1)
a) Multilayer culture
b) Transformed cells
c) Multiple growth of cells
d) Sub culturing of primary culture
1.3 Monoclonal antibodies are referred as
.
(1)
a) Magic bullets
b) Magic gun
c) Magic shots
d) Magic bomb
1.4 Salmon produce a 20 times higher efficiency
human version.
a) Ziconotide
b) Calcitonin
c) Hydroxyapatite
d) Manoalide
compared to the
(1)
1.5
Why are fish in fish farm susceptible to diseases and stress?
(1)
a) Little genetic diversity and disease resistance
b) Fish are more susceptible to stress and disease caused by bacterial and viral
pathogens
c) Large supply of fish can be wiped out quickly if disease is not controlled
d) Allthe above is correct
1.6 The production of secondary metabolites requires the use of;
(1)
(a) protoplast
(b) cell suspension
(c) meristem
(d) auxiliary buds
1.7 Synthetic seed is produced by encapsulating somatic embryo with;
(1)
(a) sodium chloride
(b) sodium alginate
(c) sodium nitrate
(d) sodium acetate
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1.8 Two methods of reproduction are represented in the diagram below.
Method A
Orginal 2
cxganism $ fos —Y
\\ 4 aje “ve{ao) |
© Booy osl
\\
’ “QO
_"
XQ
c
(Ce
| tot
E99 cet
Method B
pS
(i. aso" >
i, ietf
oOrrgiagniniaslm
’
q
\\YY.
Nudous \\
removed
CES)
C;e.
L toy)
7
Gas
fy
Lo
Ga
Ollspring
Emoryo implanted in organism 2
| Orgenis: n2
I
a
Ottsparg
How does the DNA in the offspring produced by these methods compare to the
DNA in the original organism?
a) The offspring contain half the original number of chromosomes in each
method.
b) The DNA in the offspring is genetically identical to that of the original
organism in both methods.
c) The offspring produced by method A contain twice the original number of
genes, while those produced by method B contain half the original number of
genes.
d) The number of DNA bases is less than that of the original organism in method
A, but more than the original number in method B.
QUESTION 2:
Fill in the blanks
[9]
2.1 Credit of successfully establishment the tissue on artificial culture medium goes to
German botanist
.
(1)
2.2 Name the pigment that gives shrimp their pink colour
.
(1)
2.3
involves the storing of cells at very low temperature.
(1)
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2.4
Fingerlings, sometimes called
, are usually moved into a concrete
tank called raceways, which may be indoors or outdoors.
(1)
2.5 Cell lines transformed under in vitro culture conditions give rise
. (1)
2.6
The technology Monoclonal Antibodies was developed by the scientist
(1)
2.7 AT Ill prevents
and is found in
's milk.
(1)
2.8
method uses high voltage electrical impulses for gene transfer.
(1)
QUESTION 3:
One-sentence answers
[6]
3.1 What might happen if you have high levels of cytokinin (kinetin) and low levels of
auxin (IAA) when culturing tobacco callus on nutrient agar?
(1)
3.2 In cloning the first step is the cell collected from the donor animal is placed ina
low- nutrient medium why?
(1)
3.3 What is an extraordinary new product that is used in bullet proof vests and in
suture silk for stitching wounds?
(1)
3.4 Name the scientist who developed Flavor Savr Tomatoes.
(1)
3.5 What is the application of the medical product squalamine?
(1)
3.6 How many hours the Zebrafish eggs complete embryogenesis?
(1)
QUESTION 4:
Short questions
[6]
4.1 What is the name of the drug is used to encourage hair growth and what was the
warning given to human being?
(2)
4.2 The first transgenic cow produced human protein - enriched milk. Name the cow
and the protein found in milk.
(2)
4.3 Which are the most commonly used enzymes for separating cells in a given tissue
for culture?
(2)
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QUESTION 5:
Longer questions
[44]
5.1
Explain how many members are required to sit in the Institutional Animal Care
and use committee.?
(4)
5.2 Bacterium Bacillus thuringiensis produces a toxic protein named ‘cry protein’ that is
lethal to certain insects but not to bacterium. (a) Why does this toxin not kill the
bacteria? (b) What type of changes occur in the gut of insects on consuming this
protein? (c) How has man exploited this protein for his benefit?
(4)
5.3 What are the following criteria/ characteristics are considered for efficient
development of primary cultures?
(5)
5.4 Describe the 3 morphologies of cells in culture.
(6)
5d
In the given figure, Form (A) and Form (B) represents different forms of a
proteinaceous hormone secreted by pancreas in mammals.
[8]
ci
Form ‘A’
Form'B
(a)
What type of bonding is present between chains of this hormone?
(1)
(b)
What are these form (A) and form (B). How these forms differ from each other? (3)
(c)
Explain how this hormone was produced by Eli Lilly, an American company, using
rDNA technology.
(4)
5.6
Explain the steps of agrobacterium mediated gene transformation method.
(7)
5.7
Describe the process used to create monoclonal antibodies from hybridomas. (10)
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QUESTIONS 6:
[28]
Essay questions
6.1
A businessman wishes to venture into the new business of micropropagation and
selling of high quality disease-free ornamental plants in Namibia. Advise the
businessman on how he should design/layout his laboratory and you are giving
him an advice what he will require to succeed at his new business venture.
(15)
6.2 Describe the embryonic stem cell transfer technology for the production of
transgenic mice and discuss its various applications and limitation.
(13)
THE END