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BPP702S - BIOCHEMISTRY - BIOCHEMICAL PRINCIPLES AND PRACTICE - 2ND OPP - JANUARY 2024 |
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n Am I BI A u n IVE Rs ITY
OF SCIEnCE AnDTECHnOLOGY
FacultyofHealth,Natural
ResourceasndApplied
Sciences
Schoolof NaturalandApplied
Sciences
Departmentof Biology,
ChemistryandPhysics
QUALIFICATION: BACHELOR OF SCIENCE
QUALIFICATION CODE: 07BOSC
COURSE: BIOCHEMISTRY: BIOCHEMICAL
PRINCIPLES AND PRACTICE
DATE: JANUARY 2024
DURATION: 3 HOURS
13JacksonKaujeuaStreet T: +264612072012
Private Bag13388
F: +264612079012
Windhoek
E: dbcp@nust.na
NAMIBIA
W: www.nust.na
LEVEL: 7
COURSECODE: BPP702S
SESSION: 1
MARKS: 100
SECOND OPPORTUNITY/ SUPPLEMENTARY: EXAMINATION QUESTION PAPER
EXAMINER:
MODERATOR:
PROF LAMECH MWAPAGHA
PROF PETRINA KAPEWANGOLO
INSTRUCTIONS:
1. Answer all questions on the separate answer sheet.
2. Please write neatly and legibly.
3. Do not use the left side margin of the exam paper. This must be allowed for the
examiner.
4. No books, notes and other additional aids are allowed.
5. Mark all answers clearly with their respective question numbers.
PERMISSIBLE MATERIALS:
1. Non-Programmable Calculator
This question paper consists of four (4) pages including this front page.
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Question 1
[14]
a) Calculate the pH of a mixture of 0.1 M acetic acid and 0.2 M sodium acetate. The pKa of acetic
acid is 4.75.
(3)
b) A Calculate the pH of a buffer solution that initially consists of 0.0500 M NH3 and
0.0350 M NH4+.(Ka for NH4+= 5.6 x 10-10).
(5)
c) Given the structures of lysine and the pKa values as shown below:
(6)
Lysine
pK..1= 2.18
pK.._:= 8.95
PK..1= 10.53
Determine the pl value of lysine. Show clearly how you arrive at the answer.
Question 2
[12)
a) How do cancer cells utilise anaplerosis?
(4)
b) The kinetics of facilitated diffusion can be described by the Michaelis-Menten equation. Plot a
well labelled graph depicting both the facilitated and simple diffusion curves.
(4)
c) A biosynthetic pathway proceeds from compound A to compound G in seven steps and then
branches. One branch is a three-step pathway to J, and the other is a four-step pathway to N.
Substrate C is a feed-forward activator of the enzyme that catalyzes the synthesis of G. Products J
and N are feedback inhibitors. Product N inhibits the first enzyme after the branch point in its own
pathway and Product J inhibits the second enzyme in the common pathway. Draw a diagram
showing the regulation of this metabolic pathway
(4)
Biochemistry: Biochemical Principles and Practice (BPP702S)2nd Opportunity January 2024
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Question 3
(10]
a) Outline the FOUR (4) enzyme sites that regulate gluconeogenesis
(4)
b) Given the following Fischer projection;
{6)
yH20H
9==0
H0-9-H
H-9-oH
H-9-oH
CH20H
Draw the two cyclic a and forms of this structure.
Question 4
(19]
a) Briefly describe the hormonal regulation of gluconeogenesis
{6}
b) Give the possible symptoms of deficiency of the following vitamins
{5)
I. Vitamin 82 {Riboflavin):
II. Vitamin 812:
Ill. Vitamin A {Retinal):
IV. Vitamin K {Phylloquinone):
V. Vitamin D:
c) With the aid of the fatty acyl CoA structure below, discuss the production of energy
{ATP} through the process of ~-oxidation (breakdown) of fatty acids.
(8)
0..
CH3- (Cti2l.ii:-C~- CJ-½-C-S-CoA
Fatty acy1 CoA
Biochemistry: Biochemical Principles and Practice (BPP702S)2nd Opportunity January 2024
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Question 5
[12]
a) Briefly state SEVEN (7) functions of the amino acid methionine
(7)
b) Briefly describe FIVE (5) conditions that will cause the protein to unfold, leaving only the
primary structure and rendering it non-functional.
(5)
Question 6
[18]
a) Oxidative phosphorylation is a process involving a flow of electrons through the electron
transport chain, a series of proteins and electron carriers within the mitochondrial
membrane. Briefly describe this process.
(10)
b) Lipids are known to be insoluble in water, briefly elucidate on how dietary lipid are digested,
absorbed and transported in the body.
(8)
Question 7
[15]
a) The toxicity of a drug candidate can be described in terms of the therapeutic index.
Define therapeutic index.
(4)
b) Discovery and development is one of the steps involved in drug development. Give
SEVEN(7) potential data that is gathered by researchers through conducting experiments
once they have identified a promising compound for development at this step.
(7)
c) Discuss how cholera toxin disrupts the regulation of intestinal secretion following GPCR
signalling.
(4)
THE END
Biochemistry: Biochemical Principles and Practice (BPP702S)2nd Opportunity January 2024
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