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BIO702S - BIOTECHNOLOGY - 2ND OPP - JANUARY 2024 |
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nAml BIA UnlVERSITY
OF SCIEnCE AnDTECHnOLOGY
FacultyofHealthN, atural
ResourceasndApplied
Sciences
Schoolof NaturalandApplied
Sciences
Departmentof Biology,
Chemistryand Physics
13JacksonKaujeuaStreet
Private Bag 13388
Windhoek
NAMIBIA
T; •264 612072012
F: •264 612079012
E: dbcp@nust.na
W; www.nust.na
QUALIFICATION: BACHELOR OF SCIENCE
QUALIFICATIONCODE: 07BOSC
LEVEL:7
COURSE:BIOTECHNOLOGY
COURSECODE: BIO702S
DATE: JANUARY 2024
SESSION: 1
DURATION: 3 HOURS
MARKS: 120
SECOND OPPORTUNITY/SUPPLEMENTARY: QUESTION PAPER
EXAMINER:
MODERATOR:
Prof Percy Chimwamurombe
Dr Ronnie Bock
INSTRUCTIONS
1. Answer all questions on the separate answer sheet.
2. Please write neatly and legibly.
3. Do not use the left side margin of the exam paper. This must be allowed for the
examiner.
4. No books, notes and other additional aids are allowed.
5. Mark all answers clearly with their respective question numbers.
PERMISSIBLE MATERIALS:
l. Non-Programmable Calculator
ATTACHEMENTS
1. NONE
This paper consists of 5 pages including this front pages.
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SECTIONA MULTIPLE CHOICE QUESTIONS
[20 MARKS]
Al. An example of green biotechnology is
(a) industrial antibiotics
(b) industrial chemicals
(c) industrial catalysts
(d) BT corn and pesticides
A2. Application of biotechnology procedures in agricultural field is classified as
(a) white biotechnology
(b) red biotechnology
(c) blue biotechnology
(d) green biotechnology
A3. Which of the following is a genetic vector?
(a) plasmid
(b) phage
(c) cosmid
(d) all of these
A4.The chemical knifes of DNA are
(a) ligases
(b) polymerase
(c) endo nuclease
(d) transcriptase
AS. Genetic engineering is used in
(a) gene therapy
(b) vaccine production
(c) obtaining transgenic plants
(d) all of these
AG.Thefirst hormone artificially produced by culturing bacteria is
(a) insulin
(b) thyroxine
(c) aldrekine
(d) testosterone
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A7. Which of the following process is related with the application of biotechnology
(a) fermentation
(b) germination
(c) evolution
(d) photosynthesis
AS. Biotechnology is generally being used for which of the following process
(a) production of monoclonal antibodies
(b) rain water harvesting
(c) production of GM crops
(d) both a & C
A9. GM crops means
(a) grand matter crops
(b) green metallic crops
(c) genetically modified crops
(d) growing & moving crops
AlO. DNA fragments can be separated by
(a) centrifugation
(b) gel electrophoresis
(c) boiling
(d) magnetic separation
All. PCRstands for
(a) pure chemical reaction
(b) part create reaction
(c) polymerase chain reaction
(d) partial cytoplasm rate
A12. The sequence from where replication starts
(a) origin of replication
(b) cloning site
(c) selectable marker
(d) recognition site
Al3. Which bacteria is responsible to produce Bt toxin
(a) Basilius thuringiensis
(b) Bacillus thuringiensis
(c) Bacillus tuberculosis
(d) Lacto bacillus
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A14. RNA interference is based on the principle of
(a) RNA interfere
(b) silencing of a specific mRNA
(c) prevents translation of
(d) RNA destruction
AlS. Which is first transgenic cow
(a) Dolly
(b) Eve
(c) Rosie
(d) Goldy
A16. Which is not the way to introduce alien DNA into host cells
(a) thermal shock
(b) micro injection
(c) gene gun
(d) RNA
A17. Which is the tool of recombinant DNA technology
(a) restriction endo nuclease
(b) ribosome
(c) gel-electrophoresis
(d) vectors
A18. A multiple cloning site is the same as
(a) a polylinker site
(b) an oriC
(c) a selectable marker
(d) versatile site
A19. In gel electrophoresis, DNA migrates
(a) From the negative to the positive electrode
(b) From the positive to the negative electrode
(c) Horizontally
(d) None of the above options
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A20. The DNA polymerase which is commonly used in PCRamplifications was isolated from
(a) Thermus aquaticus
(b) Agrobacterium tumifaciens
(c) Bacillus licheniformis
(d) Kosakonia species
SECTION B
(80 MARKS]
1. Describe the three types of restriction endonucleases.
(5)
2. Name and describe any three DNA modifying enzymes.
(5)
3. Draw and label a typical cloning vector.
(5)
4. Describe the process of DNA electrophoresis.
(5)
5. Describe a gene gun.
(5)
6. Describe fully the Southern blot technique.
(5)
7. Name any three cloning hosts and give the advantages and disadvantages of each of
them.
(5)
8. What are the applications of Biotechnology, give three examples in each case. (5)
9. Describe any five -Omics which you know.
(5)
10. You are the only local biotechnologist and are tasked to innovate on the development
of a transgenic banana plant with edible vaccines. How you can you do it.
(5)
11. In brief explain the process of making a transgenic plant?
(10)
12. Briefly describe the steps of DNA isolation from a plant tissue.
(10)
13. Describe the process of PCR.
(10)
SECTION C
(20 MARKS]
1. Provide possible solutions to three of the biosafety concerns of transgenic plants.
(20)
-------------------END OF EXAM I NATI ON QUESTION PAPER----------------------------
Biotechnology (B1O702S)
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