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MMB621S - MEDICAL MICROBIOLOGY 2B - 2ND OPP - JAN 2023 |
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nAmlBIA unlVERSITY
OF SCIEnCE Ano TECHnOLOGY
FACULTYOF HEALTHAPPLIEDSCIENCESAND NATURALRESOURCES
DEPARTMENTOF HEALTHSCIENCES
QUALIFICATION:BACHELOROF MEDICALLABORATORYSCIENCES
QUALIFICATIONCODE: 08BMLS
LEVEL: 6
COURSECODE: MMB621S
COURSENAME: MEDICAL MICROBIOLOGY2B
SESSION:
January 2023
PAPER:
THEORY
DURATION: 3 HOURS
MARKS:
100
SUPPLEMENTARY/SECONDOPPORTUNITYEXAMINATION QUESTION PAPER
EXAMINER(S) Mrs Fredrika Engelbrecht
MODERATOR: Ms Vanessa Tjijenda
INSTRUCTIONS
1. Answer ALL the questions.
2. Write clearly and neatly.
3. Number the answers clearly.
THIS QUESTION PAPERCONSISTSOF 4 PAGES{Excluding this front page)
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MMB621S MEDICAL MICROBIOLOGY 2B: 2nd OPPORTUNITY QUESTION PAPER
JANUARY 2023
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SECTION A
(25)
QUESTION 1
Choose the correct answer and report only the suitable letter next to the
relevant question number.
[10]
1.1 Sources of error that needs to be consider for the Citrate utilisation test
includes:
A) a light inoculum.
B) an excess of oxygen.
C) the incubation time of the test.
D) a heavy inoculum.
[1]
1.2 Mycoplasma can be defined as:
A) a gram-negative bacillus.
B) an intracellular parasite.
C) the smallest self-sufficient bacteria.
D) a common causative organism of eye infections.
[1]
1.3 Bacterial cross-resistance refers to:
A) Resistance constantly expressed.
B) One change that result in resistance to several drugs.
C) Resistance expressed when micro-organism is exposed to a specific
drug.
D) Resistance expressed by an entire population.
[1]
1.4 Durham tubes are used in microbiology in order to:
A) Interpret the ability of a specific organism to produce gas.
B) Interpret carbohydrate utilization by a specific organism.
C) Interpret the ability of a specific organism to change the pH of media.
D) Interpret whether an organism ferments or oxidize a carbohydrate.
[1]
1.5 N. asteroides and A. israelii can be distinguished by means of:
A) Gram stain reaction.
B) Ziehl-Neelson reaction.
C) Albert stain reaction.
D) Capsular stain reaction.
[1]
1.6 Bacterial antigens associated with motile organisms are:
A) K antigens
B) H antigens
C) 0 antigens
D) K and O antigens
[1]
1.7 The sample required for diagnosing the causative organism for infective
endocarditis is a:
A) nasopharyngeal swab.
B) urine sample.
C) blood culture.
D) tissue biopsy.
[1]
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MMB621S MEDICAL MICROBIOLOGY 2B: 2nd OPPORTUNITY QUESTION PAPER
JANUARY 2023
1.8 Novobiocin are used to distinguish between:
A) Staphylococcus epidermidis & Staphylococcus saprophyticus.
B) Staphylococcus aureus & Staphylococcus saprophyticus.
C) Streptococcus spp and Staphylococcus spp.
D) Staphylococcus aureus & Staphylococcus epidermidis.
[1]
1.9 Virulence factors of Streptococcus pyogenes that aids the organism to escape
host defense is(are):
A) The outermost capsule of hyaluronic acid.
B) Streptokinases A & B lyse.
C) Hyaluronidase.
D) All of the above.
[1]
1.10 The Salmonellae Vi antigen:
A) Is an acidic polysaccharide antigen that overlay the O antigen.
B) Exhibit the property of diphasic variation
C) Are fimbria antigens
D) Are determined by the complete sugar sequence.
[1]
QUESTION 2
[15]
Assess the following statements and decide whether they are true or false. Write only
the number of the question and next to it TRUE for a true statement and FALSE for a
false statement and give a reason for calling a statement FALSE.
2.1 Klebsiella pneumoniae form mucoid non-lactose fermenting colonies on the
MacConkey agar.
2.2 S. saprophyticus are the only causative organism of Toxic Shock Syndrome.
2.3 Aerotolerant organisms will grow scanty in air and good growth when
anaerobic
2.4 OrthoNitroPhenyl-~-0-Galactopyranoside test detects ~-galactosidase.
2.5 Human infections with Bruce/la melitensis are associated with cattle.
2.6 Gram negative, oxidase positive diplococci, are diagnostic for Neisseria
gonorrhoea infections in female patients.
2.7 Bubonic plaque is associated with Clostridium.
2.8 Metronidazole has no effect against many anaerobes.
2.9 The anthrax toxin causes an increase in vascular permeability resulting in
shock.
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MMB621S MEDICAL MICROBIOLOGY 2B: 2nd OPPORTUNITY QUESTION PAPER
JANUARY 2023
QUESTION 3
3.1 Categorise the following drugs by means of its mechanism of action.
A) Gentamycin
B) Metronidazole
C) Vancomycin
D) Ciprofloxacin
3.2 Differentiate, in a table form, between Bacillus anthracis, B. cereus and B.
subtilis using the following criteria:
A) Capsule,
B) Motility,
C) Catalase,
D) Lecithinase production.
(~6)
[16]
[1]
[1]
[1]
[1]
[12]
QUESTION 4
[8]
4. A patient is presenting with the following clinical symptoms from a
suspected bacterial infection:
NAUSEAV&
COLHDAND&SFEET
MUSC&UJOINPTAIN
4.1 Point out what specimen should be collected to identify the causative
pathogen?
[1]
4.2 Illustration, what you expect so see on the gram stain slide from the
specimen?
[2]
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MMB621S MEDICAL MICROBIOLOGY 2B: 2nd OPPORTUNITY QUESTION PAPER
JANUARY 2023
4.3 Suggest tests which will enable you do to identify the organism, and
give the expected results.
[4]
4.4 Predict the expected pathogen responsible for the presented clinical
symptoms.
[1]
QUESTION 5
[12]
5.1 Justify and explain why the TPHA is considered a specific serological test.
[4]
5.2 Summarize some of the mechanisms used by bacteria to resist the effects of
antibiotics and give an example for each of the mentioned mechanisms.
[8]
SECT'ION C
·(39)
QUESTION 6
6. A patient is presenting with a very sore throat. The doctor submits a throat
swab to your diagnostic medical microbiology laboratory.
[30]
A) Discuss how you would go about processing this sample and justify your
suggestions.
[6]
B) Identify the expected pathogen?
[1]
C) Illustrate, by means of a flow chart, how you would identify this organism.
[9]
6.2 Present a positive satelletism test by means of a labelled illustration and
explain the principle of the test.
[9]
6.3 Propose factors affecting the quality of the final microbiological report,
and explain why these factors affects the quality.
[5]
QUESTION 7
[9]
7.1 Discuss the diagnosing typhoid fever in the microbiology laboratory.
[9]
TOTAL:100 MARKS
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