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CLC611S - CLINICAL CHEMISTRY 2A - 1ST OPP - JUNE 2023 |
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n Am I BI A u n IVER s ITY
OF SCIEnCE Ano TECHnOLOGY
FACULTYOF HEALTH,APPLIEDSCIENCESAND NATURALRESOURCES
SCHOOLOF HEALTHSCIENCES
DEPARTMENT OF CLINICALHEALTHSCIENCES
QUALIFICATION:BACHELOROF MEDICAL LABORATORYSCIENCES
QUALIFICATIONCODE: 08BMLS
LEVEL: 6
COURSECODE: CLC611S
COURSENAME: CLINICAL CHEMISTRY2A
SESSION:
JUNE 23
PAPER:
THEORY
DURATION: 3 HOURS
MARKS:
105
FIRSTOPPORTUNITY EXAMINATION QUESTION PAPER
EXAMINER(S)
MS CARAMIA DUNAISKI
MODERATOR:
DR MUNYARADZI MUKESI
INSTRUCTIONS
1. Answer ALL the questions.
2. Write clearly and neatly.
3. Number the answers clearly.
PERMISSIBLEMATERIALS
1. CALCULATOR
THIS QUESTION PAPERCONSISTSOF 5 PAGES(Including this front page)
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SECTIONA
[20]
QUESTION 1
[10]
Analyze the statements below and provide the best possible answer. Only write the
question number and corresponding answer.
1.1
Name the basic SI unit for luminous intensity.
(1)
1.2
Explain the function of buffer solutions.
(1)
1.3
Identify the method for obtaining cerebrospinal fluid for clinical chemistry
(1)
analysis.
1.4
Give the formula which describes the measure of spread of numbers in a set of
(1)
data from its mean value.
1.5
A patient serum result for Total protein ran outside the linear range of the
instrument. The serum was diluted 1 in 10 and re-run. The reanalysed result is (1)
0.215mmol/L. What is the final patient result?
1.6
What name is given to a piece of laboratory equipment that utilizes the most
accurate means to measure controls, standards and patient samples in volumes (1)
:51ml?
1.7
Body temperature is 98.6°F. What is the temperature in Celsius?
(1)
1.8
List THREEchemical analytes most affected by IV contamination.
(3)
QUESTION 2
Outline the principles of the following analytical techniques:
2.1
Gas Chromatography/Mass Spectrometry (GC/MS)
2.2
Enzyme-Multiplied Immunoassay Technique (EMIT)
2.3
Two-Dimensional Electrophoresis
2.4
Ion-exchange chromatography
2.5
Atomic emission spectrophotometry
[10]
(2)
(2)
(2)
(2)
(2)
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SECTION B
[35]
QUESTION 3
[10]
MW: Na -23; Cl - 35.5; C-12; H -1; 0 -16
Showing all necessary working and formula explain how the following solutions are made:
3.1
A 2.15L solution of 0.9% NaCl (w/v)
(2)
3.2
1200ml of a 1.2M solution of NaOH
(2)
3.3
0.0SM HCLsolution from 250 ml of a 10M HCI
(2)
3.4
The molarity of a 800ml solution of 25g NaOH
(2)
3.5
115ml of a 0.6M solution of NaOH from SL of a 1.3M stock solution
(2)
QUESTION 4
[10]
The following are results of an evaluation for an Infectious Mononucleosis lgG Point of Care
Testing (POCT)device which is new to Namibia. Review the results (Table 1) and calculate
the following:
4.1
Positive predictive value
(2)
4.2
Negative predictive value
(2)
4.3
Diagnostic specificity
(2)
4.4
Diagnostic sensitivity
(2)
4.5
Efficiency
(2)
Table 1. Diagnostic efficacy test results
Group
People without infectious
mononucleosis
infectious mononucleosis
Negative result
920
5
Positive result
15
10
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QUESTION 5
[15]
Presented below are the results (Figure 1) of daily quality control for serum cholesterol
measurement.
The control has a mean of 200 mg/dl and standard deviation of 4.0 mg/dl.
o.,
1
234
56
7
89
10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 2S
Cooc
V-&l•ll 200 205 1,S 202 1&6 207 1!H 209 200 196 190 20I 196 207 200 205 209 197 196 198 197 195 198 199 191 197 190 202
Figure 1. Daily quality control for serum cholesterol measurement
5.1
Plot a Levey-Jennings chart of the data above (Graph paper is attached at the end
(5)
of the question paper).
5.2
Identify and interpret Westgard rules violated in this graph.
(10)
SECTIONC
[SO]
QUESTION 6
[10]
Discuss the technique of electrophoresis, measurements and quantification of protein
bands and its application in the medical laboratory.
QUESTION 7
[10]
Briefly discuss the areas in the measurement systems of spectrophotometers where errors
may arise (Denote the measurement and give a description).
QUESTION 8
[10]
The laboratory manager has asked you to introduce a new method in the laboratory.
Propose the managerial information you would consider in the method selection process.
QUESTION 9
[10]
Nephelometry is a tool used in immunology laboratories for testing levels of proteins that
are important to effective immune responses. Describe how antigen-antibody complexes,
are measured in a blood sample using nephelometry.
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QUESTION 10
[10]
A safety data sheet, material safety data sheet, or product safety data sheet is an important
component of product stewardship, occupational safety and health, and spill-handling
(10)
procedures. Outline the information which should appear MSDS(material safety data sheet)
and justify why the MSDSis crucial in the handling of chemicals.
END OF EXAMINATION
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