 |
MRT811S - METHODS IN RECOMBINANT DNA TECHNOLOGY - 1ST OPP - JUNE 2023 |
|
|
1 Page 1 |
▲back to top |
nAmlBIA unlVERSITY
OF SCIEnCE Ano TECHnOLOGY
FACULTYOF HEALTH,NATURALRESOURCESAND APPLIEDSCIENCES
SCHOOLOF NATURALAND APPLIEDSCIENCES
DEPARTMENTOF BIOLOGY,CHEMISTRYAND PHYSICS
QUALIFICATION: BACHELOROF SCIENCEHONOURS
QUALIFICATION CODE:08BOSH
LEVEL:8
COURSECODE: MRT811S
COURSENAME: METHODS IN RECOMBINANT DNA
TECHNOLOGY
SESSION:JUNE 2023
DURATION: 3 HOURS
PAPER:THEORY
MARKS: 100
EXAMINER
FIRSTOPPORTUNITYQUESTION PAPER
DR LAMECH MWAPAGHA
MODERATOR DR RONNIE BOCK
INSTRUCTIONS
1. Answer ALL the questions.
2. Write clearly and neatly.
3. Number the answers clearly.
4. All written work MUST be done in BLUEor BLACKink.
PERMISSIBLEMATERIALS
None
THIS QUESTION PAPERCONSISTSOF FOUR (4) PAGES
(Including this front page)
Page 1 of 4
|
|
2 Page 2 |
▲back to top |
,,,.
Question 1
[15]
a) Give SIX (6} Similarities between the Ti and Ri Plasmids
(6)
b) Mention THREE(3) Limitations to the particle bombardment method, compared
to Agrobacterium-mediated transformation
(3)
c) What are the steps followed in plant transformation using particle bombardment?
(6)
Question 2
[11]
a) Discuss the mode of action of small interfering RNAs (siRNAs)
(3)
b) Mention THREE(3) demerits of the Retrovirus-mediated Gene Transfer
(3)
c) Discuss in detail the mechanism behind the CRISPR/Cas9technique.
(5)
Question 3
[15]
a) The genetic code is a set of three-letter combinations of nucleotides called codons, each
of which corresponds to a specific amino acid or stop signal. DiscussTHREE(3} characteristics
of the genetic code
(6)
b) Briefly describe the following mutations;
(4)
I. Nonsense Mutation:
II. Frame shift Mutation:
Ill. Silent Mutation:
IV. Misense Mutation:
c) State FIVE (5) applications of DNA fingerprinting
(5)
Page 2 of S.,
|
|
3 Page 3 |
▲back to top |
Question 4
[18]
a) Delineate the following medical applications of transgenic animals.
(9)
I. Xenotransplanters:
II. Tools in the study of immunoglobulin genes:
Ill. Chemical Safety Testing:
b) There are several approaches scientific researchers may use in correcting faulty genes.
Briefly describe FOUR (4) of these approaches
(4)
c) Gene therapy is a medical approach that treats or prevents disease by correcting the
underlying genetic problem. What are some of the risks envisioned in Gene Therapy?
(5)
Question 5
[13]
You are given a plasmid. In order to map this plasmid you set up a series of restriction
digests and obtain the following results using agarose gel electrophoresis.
-1.21,.'b
3.SKb
3.21,.'b
2.SKb
2.0Kb
l.SKb
1.0Kb
---
•:•l\\1a1nd l\\12arc DNAmarkers.
800bp
700bp
600bp
500bp
400bp
300bp
200bp
lOObp
Lane
1
2
3
4
5
6
Digest
EcoRIand Natl
Natl and Hindlll
Hindlll and Xbal
EcoRIand Hindlll
Hind Ill
Xbal and EcoRI
Size of fragments in bp
4200,800
3200,1500,300
2500,1500,1000
3500,1000,500
3500, 1500
3500,1500
Page3 of 5
|
|
4 Page 4 |
▲back to top |
a) What is the approximate size of the plasmid?
(2)
b) Add the Notl, Hindlll, Xbal restriction sites onto the plasmid map shown below. On your map
give the distances between each of the restriction sites.
(7)
EcoRI
c) Mention FOUR (4) factors that make a Vector ideal for cloning.
(4)
Question 6
[10)
a) State FOUR (4) factors affecting migration of nucleic acid in gel electrophoresis
(4)
b) DiscussTHREE(3) analytical applications of gel electrophoresis?
(3)
c) Discuss the one-Channel DNA microarray hybridisation approach.
(3)
Page 4 of 5
|
|
5 Page 5 |
▲back to top |
f
!
Question 7
[18)
a) Briefly describe the functions of the following RNA's:
(10)
I. Messenger RNA (mRNA)
II. Ribosomal RNA (rRNA):
Ill. Transfer RNA (tRNA)
IV. Small nuclear RNA (snRNA)
V. microRNA (miRNA)
b) Briefly delineate the Four (4) levels of the Protein Structure
(4)
c) Describe FOUR (4) applications of Proteomics
(4)
THE END
Page 5 of 5