BIO702S - BIOTECHNOLOGY - 2nd Opp - Jan 2025


BIO702S - BIOTECHNOLOGY - 2nd Opp - Jan 2025



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nAm I BIA UnlVERSITY
- OF SCIEnCE TECHnOLOGY
FacultyofHealth,Natural
ResourceasndApplied
Sciences
Schoolof NaturalandApplied
Sciences
Departmentof Biology,
Chemistryand Physics
QUALIFICATION : BACHELOR OF SCIENCE
QUALIFICATION CODE: 07BOSC
COURSE: BIOTECHNOLOGY
DATE: JANUARY 2025
DURATION: 3 HOURS
13JacksonKaujeuaStreet
Private Bag13388
Windhoek
NAMIBIA
T: +264612072012
F: +264612079012
E: dbcp@nust.na
W: www.nust.na
LEVEL: 7
COURSECODE: BIO702S
SESSION: 1
MARKS: 120
SECOND /SUPPLEMENTARY OPPORTUNITY: QUESTION PAPER
EXAMINER: Prof Percy Chimwamurombe
MODERATOR: Dr Jean Damascene Uzabakiriho
INSTRUCTIONS
1. Answer all questions on the separate answer sheet.
2. Please write neatly and legibly.
3. Do not use the left side margin of the exam paper. This must be allowed for the examiner.
4. No books, notes and other additional aids are allowed.
5. Mark all answers clearly with their respective question numbers.
PERMISSIBLE MATERIALS:
1. Non-Programmable
ATTACHEMENTS
1. NONE
Calculator
This paper consists of 2 pages including this front pages.

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SECTIONA
[80 MARKS]
1. Describe the steps of DNA extraction from a plant leaf.
(5)
2. Describe a technique of plant regeneration that you know and its
application in producing genetically modified plants.
(5)
3. Describe any five applications of the technique of biopharming.
(10)
4. Distinguish between biotechnology and biosafety.
(5)
5. Explain the importance of public awareness on biotechnology and biosafety?
(5)
6. Describe any five applications of the PCRtechnology
(5)
7. Describe the process of Western blotting.
(5)
8. Describe the process of Northern Blotting.
(5)
9. Describe the technique of sited-directed mutagenesis.
(10)
10. Draw any similarities and differences between RAPDsand RFLPs
(10)
11. State the details of how "Golden rice" was produced?
(10)
12. Explain the use of gene mining in prospecting for genes to produce a GMO.
(5)
SECTIONB
[40 MARKS]
1. Describe using examples, the application of biotechnology in
forensics regarding contemporary issues of society.
(20)
2. In detail, describe a risk assessment procedure for evaluating the environmental safety of a
transgenic plant.
(20)
--------END OF EXAMINATION QUESTION PAPER------
Biotechnology (8107025)
2nd Opportunity January 2025
2