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PAB811S - PLANT AND ANIMAL BIOTECHNOLOGY - 1ST OPP - JUNE 2022 |
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NAMIBIA UNIVERSITY
OF SCIENCE AND TECHNOLOGY
FACULTY OF HEALTH, APPLIED SCIENCES AND NATURAL RESOURCES
DEPARTMENT OF NATURAL AND APPLIED SCIENCES
QUALIFICATION: BACHELOR OF SCIENCE HONOURS
QUALIFICATION CODE: O8BOSH
COURSE CODE: PAB811S
LEVEL: 8
COURSE NAME: PBLIAONTTECAHNNDOLAONGIYMAL
SESSION: JUNE 2022
DURATION: 3 HOURS
PAPER: THEORY
MARKS: 100
EXAMINATION QUESTION PAPER
EXAMINER(S) | DR JEYA KENNEDY
MOoperRATOR;: | DRJUAN VORSTER
INSTRUCTIONS
Write clearly and neatly
Number the answers clearly
All written work MUST be done in blue or black ink
No books, notes and other additional aids are allowed
Mark all answers clearly with their respective question
Draw diagrams wherever necessary
numbers
PERMISSIBLE MATERIALS
None
THIS QUESTION PAPER CONSISTS OF 4 PAGES
(Including this front page)
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QUESTION 1:
Multiple choices
[6]
1.1
Hormone pair required for a callus to differentiate are;
(a) auxin & cytokinins
(b) auxin & gibberellin
(c) cytokinins & abscisic acid
(d) all of them
1.2
Part of plant used for culturing is called;
(a) scion
(b) explant
(c) stock
(d) callus
13
DMSO (Dimethyl sulfoxide) is used as;
(a) gelling agent
(b) alkalating agents
(c) chelating agent
(d) cryoprotectant
1.4 Steps in a reproductive process used to produce a sheep with certain traits are listed
below.
Step 1 — The nucleus was removed from an unfertilized egg taken from
sheep A. Step 2 — The nucleus of a body cell taken from sheep B was then
inserted into this unfertilized egg from sheep A.
Step 3 — The resulting cell was then implanted into the uterus of sheep C.
Step 4 — Sheep C gave birth to sheep D.
Which sheep would be most genetically similar to sheep D?
(a) sheep A, only
(b) sheep B, only
(c) both sheep A and B
(d) both sheep A and C
1.5 Hybridoma cells have an application to produce; (1)
(a) Antigens
(b) Antibodies
(c) Cancer cells
(d) Cell lines
1.6 Name the pigment that gives shrimp their pink colour.
(a) prialt
(b) eribulin
(c) yondelis
(d) astaxanthin
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QUESTION 2:
Fill in the blanks
[5]
2.1
Credit of successfully establishment the tissue on artificial culture medium goes to
German botanist
.
(1)
2.2
method of gene transfer requires skilled personnel.
(1)
2.3 Differentiation of roots, shoots and leaves from callus is called
. (1)
2.4 — Activated charcoal is used in nutrition media to
.
(1)
2.5
are those cell lines which have a limited life span, and they grow
through a limited number of cell generations.
(1)
QUESTION 3:
One sentence
[5]
3.1 Which is the most widely used vector for producing Bt crops.
(1)
3.2
Name the scientist who developed pest resistant plant by using RNA interference
technique.
(1)
3.3 Name the compound used as cryoprotectants.
(1)
3.4 What might happen if you have low levels of cytokinin (kinetin) and high levels of
auxin (IAA) when culturing tobacco callus on nutrient agar?
(1)
3.5
What type of LAFs are present in plant tissue culture laboratory?
(1)
QUESTION 4:
Longer questions
[56]
4.1 Why is apical meristems with low concentration of viruses?
(3)
4.2 Describe three artificial types of media used in animal cell culture.
(6)
4.3
Explain the different stages involved in micropropagation.
(5)
4.4
Asaresearch scientist you have been tasked to generate genetic transformation
of plants by ‘Wounded explant’ by using Agrobacterium-mediated. Briefly state
SIX (6) steps you would consider when designing this project.
(6)
4.5 Explain the mechanism of RNA interference.
(7)
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4.6
Why are zebrafish commonly being used to test new human drugs?
(5)
4.7 What is the difference and similarities between cold trypsinization and warn
trypsinization?
(5)
4.8 David is given the T-flask of cell culture which grow as monolayer adhering to
the flask surface that is fully confluency, now his lecture told him, the flask can
be split or divided to seed 2-4 new flasks depending on the vigour of the
cell growth. He came to you for help what advice do you give him to follow the
procedure.
(10)
4.9 You are given a task in the lab to make a medium for callus culture, what type of
media you are going to use and briefly mention the composition you are going to
add in the media. How will you induce callus from it and the advantages of callus
culturing?
(9)
QUESTION 5:
Essay questions
[238]
5:1 As a NUST science student a small-scale farmer came to you for advice about
triploid grass carp and transgenic fish in aquaculture. He wants to know what
triploid species are and how to create triploid species? What will be the uses and
issues regarding triploid grass carp? What is the difference between transgenic
fish and triploids? Can you advice the farmer from the theory point of view of
what have you learned?
(13)
5.1 As a research scholar you have been tasked to write the procedure of isolation of
protoplasts culture from the plant leaf cells. Briefly state the necessary steps in order
for you to consider when writing this project and mention its application.
(15)
THE END